The show, which regularly achieves an audience of over 2 million viewers, sees members of the public take along 3 antiques and collectables they might be interested in selling.
Once valued, the owner and a team of experts decide whether an object should go forward for auction. If the item is chosen it is included in a sale a few weeks later - and hopefully when the auctioneer's hammer falls its owners make a tidy sum.
The Flog It crew filmed at the art gallery in October 2015, with the results being shown on BBC One on Monday 7 November and Friday 18 November (both start at 4.30pm), and in other future editions.
Presenter Paul Martin said: "I'm delighted we took Flog It back to Wolverhampton; it's a great city which the show first visited in 2006 when we filmed at the Civic Hall.
"I'm looking forward to revealing some of the art gallery's fascinating history to our viewers."
Councillor John Reynolds, City of Wolverhampton Council's Cabinet Member for City Economy, added: "We are delighted Paul Martin and the Flog It team came back to Wolverhampton with their hit BBC One show.
"Wolverhampton Art Gallery is a fantastic location for a valuation day, and it is great some of the gallery's treasures will be shown off to a national audience."
The show has uncovered items such as a rare Aboriginal Broad Shield that had been kept hidden away in a Flog It viewer's wardrobe and was brought along to a valuation day in North Lincolnshire before going on to sell at auction for £30,000. An unusual Royal Doulton Spook figurine in Blackpool, which had been bought at a car boot sale for £2, went on to sell at auction for £5,000.
- released: Friday 4 November, 2016